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Burns

Burns

Human skin seems to be simply external coverage of the human body. This statement is not completely true, as human skin, in addition to its protective function from the external environment, does have a range of other functions such as mechanical barrier, external immune barrier, participation in thermoregulation, excretion of sweat and sebum, sensory functions, and identification functions for the human being. Skin is the largest organ of the human body, as its average surface in adult persons is about 2 m2 and its total mass is about 5 kg. An extensive burn injury affecting more than 20% of the body surface area, can lead to temporary, or permanent dysfunction or failure of the skin organ which could become a life threatening situation.

 

Pleuropneumonia

Pleuropneumonia

Community-acquired pneumonia (CAP) is a serious public health concern and a major cause of mortality and morbidity. Despite advances in antimicrobial therapies, microbiological diagnostic tests and prevention measures, pneumonia remains the main cause of death from infectious disease in the world. An important reason for the increased global mortality is the impact of pneumonia on chronic diseases, along with the increasing age of the population and the virulence factors of the causative microorganism.
In 2013, the Global Burden of Disease Study based on data from 188 countries around the world, reported that lower respiratory tract infection was the second most common cause of death. In Europe, mortality rates for CAP vary widely from country to country, ranging from <1% to 48%.

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author: Ivana Paraničová | UPJŠ LF v Košiciach | discipline: Internal Medicine, Respiratory Medicine | category: Casuistics in image | keywords: J18.9 Pneumonia, pneumonia, pleuritis, streptococcus pneumoniae | attachment: 7 | viewed: 131x | published on: 1.1.2018 | last modified on: 16.4.2018

Treatment of overjet by retraction of frontal teeth to temporary anchorage devices (TADs)

Treatment of overjet by retraction of frontal teeth to temporary anchorage devices (TADs)

Increased overjet may be caused by excessive proclination of upper incisors with prominent maxilla or on the other hand by microgenia or mandibular retrognathism. The first malocclusion is in adult patients usually corrected by extraction of first premolars and successive retraction of frontal teeth. The second malocclusion in adult patients is correctly treated with orthognathic surgery.
When treating with retraction of frontal teeth, it is essential to assess the amount of anchorage needed. If the movement of distal teeth mesially is restricted, the absolute anchorage secured by TADs is needed. TADs are placed into the bone in the right position. Closed coil springs are placed inbetween the frontal segment and TADs on each side, thus retracting frontal segment without any movement of posterior teeth.

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author: Renáta Urban, Vladimíra Schwartzova | UPJŠ LF v Košiciach | discipline: Dentistry | category: Casuistics in image | keywords: treatment, overjet, retraction of frontal teeth | attachment: 4 | viewed: 135x | published on: 31.12.2017 | last modified on: 16.4.2018

A two stage non extraction treatment of distoocclusion with proclination of upper incisors (AII/1)

A two stage non extraction treatment of distoocclusion with proclination of upper incisors  (AII/1)

A two stage treatment of distoocclusion with proclination of upper incisors (AII/1) is very efficient in correctly indicated cases and the timing of the treatment is essential. During the growth spurt the removable functional appliance is placed in the mouth (in this case bionator) to provide the bite shift to AI class and to raise the deep bite by correctly trimmed functional appliance. The second phase of the treatment with fixed appliance is reduced and only the anomalies of the position of individual teeth are corrected.

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author: Renáta Urban, Vladimíra Schwartzova | UPJŠ LF v Košiciach | discipline: Dentistry | category: Casuistics in image | keywords: distoocclusion, proclination of upper incisors | attachment: 6 | viewed: 99x | published on: 31.12.2017 | last modified on: 16.4.2018

Home mechanical ventilation in a patient with Duchenne muscular dystrophy

Home mechanical ventilation in a patient with Duchenne muscular dystrophy

Duchenne muscular dystrophy (DMD) is a hereditary progressive neuromuscular disorder that is inherited in X-linked recessive pattern. Mutation of dystrophin gene results into a loss of integrity and function of muscular fibres and their replacement by adipose and connective tissue. Muscle degeneration leads to motoric impairment and loss of walk around 12th year of age. Myocardium is also frequently affected. Respiratory muscles impairment and other complications lead to respiratory failure. If left untreated, death occurs around 20th year of life, usually due to cardiac or respiratory causes. Respiratory insufficiency development is treated by mechanical ventilation support.

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Posterior wall myocardial infarction

Posterior wall myocardial infarction

Isolated posterior myocardial infarction is occurring in 3-11% of all infarctions. ST - segment depression in leads V1-V3 suggests myocardial ischaemia, especially when the terminal T - wave is positive (ST - segment elevation equivalent), and confirmation by concomitant ST-segment elevation ≥ 0,5mm recorded in leads V7-V9 should be considered as a mean to identify posterior myocardial infarction. Emergent coronary angiography and percutaneous coronary intervention of the infarct - related artery is indicated.

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author: Marianna Vachalcová | UPJŠ LF v Košiciach | discipline: Cardiology, Angiology, Internal Medicine | category: Casuistics in image | keywords: I21.2 Acute transmural myocardial infarction of other sites, myocardial infarction | attachment: 11 | viewed: 124x | published on: 8.12.2017 | last modified on: 30.4.2018

Cardiac tamponade due to cardiac metastases

Cardiac tamponade due to cardiac metastases

Cardiac tamponade is a clinical syndrome caused by the accumulation of fluid or gas in the pericardial space, resulting in reduced ventricular filling and therefore decreased cardiac output (Figure 1). The condition is a medical emergency with resultant cardiogenic shock and death if not treated immediately. The presented case report describes a 63-year-old oncological patient who developed cardiac tamponade with the need of pericardiocentesis.

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author: Ivana Trojová | UPJŠ LF v Košiciach | discipline: Cardiology, Angiology, Internal Medicine | category: Casuistics in image | keywords: Cardiac tamponade, Cardiac tamponade | attachment: 5 | viewed: 168x | published on: 4.12.2017 | last modified on: 30.4.2018

The Use of the Er:YAG Laser 2940 nm in complex caries removal

The Use of the Er:YAG Laser 2940 nm in complex caries removal

Significant pain reduction and lack of the vibrations give advantage to LASER cavity preparation. The ideal treatment should be fast, painless and effective. Discomfort of the patient and tendency to protect hard dental tissues can lead to insufficient debridement of the carious lesion, inadequate obturation and failure of the restoration. A carious lesion is usually removed using hand instruments and rotary burs.
Understanding of laser physics and laser-tissues interaction for specific wavelength is required to predict the accomplishment of laser treatment.

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author: Jana Ohlasová, Vladimíra Schwartzová, Silvia Timková | UPJŠ LF v Košiciach | discipline: Biophysics, Dentistry | category: Casuistics in image | keywords: Er:YAG laser, caries, ablation, Er:YAG laser, caries, ablation | attachment: 8 | viewed: 220x | published on: 1.12.2017 | last modified on: 16.4.2018

Constrictive pericarditis, reoperation

Constrictive pericarditis, reoperation

The case report describes the hospitalization of a 50-year-old man who underwent surgery for a constrictive pericarditis at a younger age. Current CT and ultrasonographic findings confirmed the recurrence of this disease. Partial pericardectomy was performed with the release of the left ventricular diaphragm wall in particular. The postoperative period was complicated by ventricular fibrillation with high speed response of chambers. After successful cardioversion, the patient was released to outpatient care on the seventh postoperative day.

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author: Tomáš Toporcer | UPJŠ LF v Košiciach | discipline: Cardiology, Angiology, Surgery, Traumatology and Orthopaedics | category: Casuistics in image | keywords: Constrictive pericarditis, atrial fibrillation | attachment: 9 | viewed: 182x | published on: 22.11.2017 | last modified on: 16.4.2018

Mitral valve reconstruction because of Barlow's disease with mitral insufficiency, perioperative SAM (systolic anterior motion)

Mitral valve reconstruction because of Barlow's disease with mitral insufficiency, perioperative SAM (systolic anterior motion)

The case report describes the hospitalization of a 26 year old male with M. Barlow. The mitral valvuloplasty was performed. However, the peroperative ultrasonographic finding documented prolapse of the anterior cusp of the mitral valve into the outflow tract of left ventricle. The surgery was supplemented with a suture of the A2-PA segments of mitral valve. The subsequent finding was satisfactory. No more serious postoperative complications were noted. The patient was admitted to outpatient care on the eighth postoperative day.

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author: Tomáš Toporcer | UPJŠ LF v Košiciach | discipline: Cardiology, Angiology, Surgery, Traumatology and Orthopaedics | category: Casuistics in image | keywords: M. Barlow, mitral valve insufficiency | attachment: 14 | viewed: 226x | published on: 22.11.2017 | last modified on: 16.4.2018

Ascending aorta replacement and repair of aortic valve because of ascending aorta aneurysm and bicuspid aortic valve with regurgitation

Ascending aorta replacement and repair of aortic valve because of ascending aorta aneurysm and bicuspid aortic valve with regurgitation

The case report describes the hospitalization of a patient with an ascending aortic aneurysm and a bicuspidal aortic valve. Valvular surgery with an annular annuloplasty ring implant was performed. At the same time, replacement of the ascending aorta was performed. There were no more serious complications in the postoperative period and the patient was released to outpatient care on the eighth postoperative day.

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author: Tomáš Toporcer | UPJŠ LF v Košiciach | discipline: Cardiology, Angiology, Surgery, Traumatology and Orthopaedics | category: Casuistics in image | keywords: Aortic valve surgery, bikuspidal aortic valve | attachment: 18 | viewed: 157x | published on: 22.11.2017 | last modified on: 16.4.2018

Appendicitis acuta and Meckel diverticulum

Appendicitis acuta  and Meckel diverticulum

Appendicitis is one of the most common reasons for surgery and emergency hospitalization due to acute abdominal pain. Accurate diagnosis of acute appendicitis and subsequent indication for surgery can prevent complications. Conversely, the surgeon´s decision to operate with the aim of avoiding the complications of a late surgical intervention in positive appendicitis cases leads to negative appendectomy in 10,5% of the cases.

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author: Martina Vidová Uğurbaş, Lucia Sukovská Lakyová | UPJŠ LF v Košiciach | discipline: Surgery, Traumatology and Orthopaedics | category: Casuistics in image | keywords: K35 Acute appendicitis, Q43.0 Meckel's diverticulum, appendicitis, diagnostic, surgical treatment | attachment: 5 | viewed: 165x | published on: 13.11.2017 | last modified on: 16.4.2018

Journey of a swallowed wooden stick to the liver

Journey of a swallowed  wooden stick to the liver

Hepatic abscess due to perforation of the gastrointestinal tract caused by ingested foreign bodies is a rare complication. Pre-operative diagnosis is difficult because of negative anamnesis of the accident and symptoms are usually non-specific. Problems can occur after months or even years after incident. CT is the most suitable imaging method for diagnostic.
The authors report a case of 57 year old man who was admitted with liver abscess, who underwent surgery mid-year after swallowing of wooden stick.

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author: Martina Vidová Uğurbaş, Lucia Sukovská Lakyová | UPJŠ LF v Košiciach | discipline: Surgery, Traumatology and Orthopaedics | category: Casuistics in image | keywords: foreign material, perforation, infection, foreign material, perforation, infection | attachment: 2 | viewed: 130x | published on: 28.10.2017 | last modified on: 16.4.2018

B lymphocytes and plasma cells

B lymphocytes and plasma cells

B lymphocytes are principal cells that mediate humoral adaptive immunity. After their maturation in the bone marrow, B cells enter peripheral lymphoid tissues, which are the sites of interaction with foreign antigens. Production of antibodies is initiated by the interaction of antigens with a small number of mature B cells specific for each antigen. An antigen binds to the membrane receptors on specific B cells and initiates a series of responses that lead to two principal changes: cell proliferation resulting in expansion of the clone, and differentiation to either plasma cells actively secreting antibodies or to memory cells.

Key words: B cells, subsets of B cells, memory B cells, plasma cells

 
author: Milan Buc | LF UK v Bratislave | discipline: Immunology, Allergology | category: Lectures, Presentations | keywords: B cells, B cell subsets, memory B cells, plasma cells | attachment: 1 | viewed: 2134x | published on: 6.3.2017

Pediatric oncology for medical students – e-learning programme

Pediatric oncology for medical students – e-learning programme

The electronic textbook "Pediatric oncology for medical students” provides a comprehensive overview of the age-specific differences between pediatric tumors and tumors of the adult age, importance of the age for diagnosis of cancer in childhood, factors influencing treatment decisions and the risks of anticancer therapy.. General section of the textbook provides a concise overview of the fundamental differences between pediatric and adult oncology, age specific clinical presentation of of the most common pediatric tumors, basic diagnostic procedures and treatment modalities of malignant diseases and last but not least the importance of organisation and centralisation of pediatric oncology on national and international level. Special section of the textbook offers an overview of the common and rare malignant tumors in children from epidemiology, etiology, clinical presentation, diagnostic procedures, differential diagnosis to treatment modalities and prognosis. The...

 
author: Viera Bajčiová, Pavel Mazánek, Karel Zitterbart | LF MU | discipline: Oncology, Radiation Therapy, Paediatrics, Neonatology | category: Multimedia, Educational websites | keywords: Children, cancer, pediatric oncology, age specific differences | attachment: 1 | viewed: 1438x | published on: 25.1.2016 | last modified on: 14.9.2016
 

Autoimmune hepatitis

Autoimmune hepatitis

Autoimmune hepatitis is chronic hepatitis associated with hypergammaglobulinaemia and the presence of circulating autoantibodies. In most cases it responds favorably to immunosuppressive therapy. Diagnosis is challenging, in general, all other possible causes of chronic hepatitis should be excluded. The case report describes a case of type 1 autoimmune hepatitis in a 69-year-old patient.

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Aortic dissection

Aortic dissection

A breach in the integrity of the aortic wall allows arterial blood to enter the media, which is then split into two layers, creating a “false lumen“ alongside the existing or “true lumen“. The primary event is often a spontaneous or iatrogenic tear in the intima of the aorta. Disease of the aorta and hypertension are the most important aetiological factors. The aortic dissection is a relatively rare condition, but it may rupture and have fatal consequences. The case report describes a dissection of the descending aorta in a 55-year-old patient.

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author: Katarína Demková, Jana Grofčíková | UPJŠ LF v Košiciach | discipline: Cardiology, Angiology, Internal Medicine | category: Casuistics in image | keywords: aortic dissection, CT aortography | attachment: 2 | viewed: 94x | published on: 1.1.2018 | last modified on: 30.4.2018

Combined orthodontic-surgical treatment of impacted teeth

Combined orthodontic-surgical treatment of impacted teeth

Impaction or impacted tooth is a tooth that didn’t erupt into its position in the right time of his physiological eruption, is covered by bone and requires treatment. After precisely locating the position of impacted tooth, the appropriate orthodontic appliance is adapted, the space for impacted tooth is created. With surgical exposure of the tooth, the crown is uncovered and eruption chain is adapted. The pull is activated by elastic thread or other auxiliaries such as Kilroy spring or Monkey hook, that help to move the tooth into required position.

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author: Renáta Urban, Vladimíra Schwartzova | UPJŠ LF v Košiciach | discipline: Dentistry | category: Casuistics in image | keywords: orthodontic-surgical treatment, impacted teeth | attachment: 4 | viewed: 121x | published on: 31.12.2017 | last modified on: 16.4.2018

Total atelectasis of the right lung due to bronchogenic carcinoma

Total atelectasis of the right lung due to bronchogenic carcinoma

Despite the emerging developments in the diagnosis and treatment of lung cancer in recent years, it is still the most lethal among all cancers. Atelectasis is a common condition with lung cancer. Central bronchogenic tumors often induce atelectasis due to endobronchial obstruction but less frequently due to compression of tumor or pleural effusion. The presented case describes case of a patient, who was repeatedly admitted to the Department of Pneumology and Phtiseology due to bronchogenic carcinoma with the future release of it´s complications including obstructive atelectasis of the right lung.

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author: Ivana Trojová | UPJŠ LF v Košiciach | discipline: Oncology, Radiation Therapy, Respiratory Medicine | category: Casuistics in image | keywords: atelectasis, bronchogenic carcinoma | attachment: 4 | viewed: 91x | published on: 23.12.2017 | last modified on: 30.4.2018

Obesity hypoventilation syndrome

Obesity hypoventilation syndrome

Obesity hypoventilation syndrome (OHS) is defined as the combined presence of obesity (BMI > 30kg/m2) with awake arterial hypercapnia (pCO2 > 6.0 kPa / 45 mmHg) in the absence of other causes of hypoventilation. The exact prevalence of OHS in the general population remains unknown, and most prevalence data describe subjects with obstructive sleep apnoea, wherein its prevalence has been estimated to range from 10% to 38% in different groups. On the other hand, patients with OHS often suffer from sleep-related breathing disorders. Prevalence of obstructive sleep apnoea may reach up to 90-95% in OHS patients.

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CYTOCHROME P-450: genetic and population aspects

CYTOCHROME P-450: genetic and population aspects

Need of carrying out a pharmacological genotyping for providing the individualized pharmacotherapy is shown in the monograph based on the analysis of own and literary data of the central link of drug pharmacokinetics– systems of cytochrome P-450, taking into account its genetic polymorphysm. The monograph is intended for the clinical pharmacologists, geneticists and experts dealing with this problem.

Mavlyanov I.R., Ashirmetov A.Kh., Mavlyanov Z.I., Jarilkasinova G.J.: CYTOCHROME P-450: genetic and population aspects, Pavol Jozef Šafárik University in Košice, Košice 2017, ISBN 978-80-8152-526-1.

 
author: Iskander Rahimovich Mavlyanov, Abdurashid Khamidovich Ashirmetov, Zafar Iskandarovich Mavlyanov, Gavhar Januzakovna Jarilkasinova | UPJŠ LF v Košiciach | discipline: Biology, Pharmacology, Medical Chemistry and Biochemistry | category: Educational works | keywords: cytochrome P-450 | attachment: 2 | viewed: 143x | published on: 5.12.2017 | last modified on: 23.3.2018

Pathological physiology. Selected chapters.

Pathological physiology. Selected chapters.

The textbook Pathological physiology: selected chapters contains chapters of general and special pathological physiology that explain the causes and mechanisms of disease and pathological processes in synergy with their future clinical application. Simple case reports in the chapters point to the clinical relevance of the knowledge gained in the study of pathological physiology and connect the theoretical and clinical disciplines to be addressed by students in subsequent years of study. It supports the ability of students to think logically and combine knowledge they have already obtained by study of different theoretical subjects.

 
author: Jana Plevková, Ján Hanáček,Miloš Tatár, Mariana Brozmanová,Tomáš Buday | JLF UK v Martine | discipline: Physiology and Pathophysiology | category: Educational works | keywords: pathophysiology, mechanisms, disease, case reports, pathogenesis | attachment: 3 | viewed: 293x | published on: 3.12.2017 | last modified on: 4.12.2017

Mitral valve replacement and MAZE procedure because of mitral valve stenosis and paroxysmal atrial fibrillation

Mitral valve replacement and MAZE procedure because of mitral valve stenosis and paroxysmal atrial fibrillation

The case report describes the hospitalization of a 63-year-old patient admitted to the Department of heart surgery for mitral valve stenosis with diagnosed atrial fibrillation. The patient underwent mitral valve replacement and MAZE procedure. At the postoperative period, paroxysm of atrial fibrillation was successfully detected with a successful version. On the tenth postoperative day, the patient was placed in outpatient care.

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author: Tomáš Toporcer | UPJŠ LF v Košiciach | discipline: Cardiology, Angiology, Surgery, Traumatology and Orthopaedics | category: Casuistics in image | keywords: Mitral valve stenosis, paroxysmal atrial fibrillation | attachment: 15 | viewed: 250x | published on: 22.11.2017 | last modified on: 16.4.2018

Aortocoronary bypass

Aortocoronary bypass

The case report describes the hospitalization of a 58 year old male admitted to our department for documented ischemic heart disease with affection of three coronary arteries. The patient underwent a cardiac surgery with the formation of one bypass with arterial graft and one bypass with venous graft. In the postoperative period, fibrillation of atrial fibrillation with successful cardioversion was noted. The patient was admitted to outpatient care on the seventh postoperative day.

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author: Tomáš Toporcer | UPJŠ LF v Košiciach | discipline: Cardiology, Angiology, Surgery, Traumatology and Orthopaedics | category: Casuistics in image | keywords: Ischemic heart disease, aortocoronary bypass | attachment: 10 | viewed: 148x | published on: 22.11.2017 | last modified on: 16.4.2018

Aortic valve replacement because of aortic valve stenosis, repair of tricuspid valve because of regurgitation

Aortic valve replacement because of aortic valve stenosis, repair of tricuspid valve because of regurgitation

The case study describes the hospitalization of women with aortic valve stenosis and secondary regurgitation of the tricuspid valve. The patient underwent aortic valve replacement by bioprosthesis and repair surgery of the tricuspid valve. The postoperative course was complicated by pneumohorax on the right side. Drainage was performed. The patient was placed in outpatient care on the eighth postoperative day.

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author: Tomáš Toporcer | UPJŠ LF v Košiciach | discipline: Cardiology, Angiology, Surgery, Traumatology and Orthopaedics | category: Casuistics in image | keywords: Aortic valve stenosis, tricuspid valve insufficiency, pneumothorax | attachment: 13 | viewed: 172x | published on: 22.11.2017 | last modified on: 16.4.2018

Management of breast cancer

Management of breast cancer

Breast cancer is the most frequently diagnosed life-threatening cancer in women and the leading cause of cancer death among them worldwide. It includes a heterogeneous collection of diseases with various histologically defined subsets, clinical presentations, responses to treatment and outcomes. We describe a case of female patient with ductal breast carcinoma.

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author: Martina Vidová Uğurbaş, Lucia Sukovská Lakyová | UPJŠ LF v Košiciach | discipline: Surgery, Traumatology and Orthopaedics | category: Casuistics in image | keywords: D05 Carcinoma in situ of breast, carcinoma of breast, diagnostic, treatment | attachment: 15 | viewed: 149x | published on: 19.11.2017 | last modified on: 16.4.2018

Acute abdomen - gallstone ileus

Acute abdomen - gallstone ileus

Gallstone ileus is an uncommon entity, which accounts for 1–4% of all presentations to hospital with small bowel obstruction and for up to 25% of all cases in patients over 65 years of age. Despite medical advances, gallstone ileus is still associated with high rates of morbidity and mortality. The management of gallstone ileus remains controversial. The clinical signs and symptoms of gallstone ileus are usually non-specific, contributing to a delay in diagnosis. We present our case admitted to the hospital with gallstone ileus.

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author: Martina Vidová Uğurbaş, Lucia Sukovská Lakyová | UPJŠ LF v Košiciach | discipline: Surgery, Traumatology and Orthopaedics | category: Casuistics in image | keywords: biliary ileus, surgical treatment, biliary ileus, surgical treatment | attachment: 5 | viewed: 132x | published on: 28.10.2017 | last modified on: 16.4.2018

Management of pilonidal sinus

Management of pilonidal sinus

A pilonidal sinus is a sinus track which commonly contains hair. It occurs under the skin between the buttocks at a short distance above the anus. Most cases occur in young male adults. The most commonly used therapy is surgery including incisions, marsupialization, primary midline closure and skin flaps. We present two cases treated with different surgical techniques.

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author: Martina Vidová Uğurbaş, Lucia Sukovská Lakyová | UPJŠ LF v Košiciach | discipline: Surgery, Traumatology and Orthopaedics | category: Casuistics in image | keywords: sinus pilonidalis, surgical treatment, sinus pilonidalis, surgical treatment | attachment: 9 | viewed: 139x | published on: 27.10.2017 | last modified on: 16.4.2018

E-LEARNING COURSE: DONATION AND HARVEST OF CELLS AND TISSUES

E-LEARNING COURSE: DONATION AND HARVEST OF CELLS AND TISSUES

The design of the e-learning course: Donation and Harvest of cells and tissues“ is presented. This course represents more advanced updated and enlarged English version of the original e-learning course focused exclusively on the issues of cell and tissue donation and harvest.
The course covers the topic Donation and harvest of cells and tissues and is divided into 2 parts. A test consisting of 5 questions follows each part. If the student answers correctly at least 3 questions he/she is allowed to pass to the next part and/or final test consisting of 15 questions. It is necessary to answer correctly at least 10 questions to complete the course.
The third topic is represented by a database for uploading seminar theses.
The previous experience showed that the e-learning course could serve as a useful support of the elective subject „Basis of Harvest and Preservation of Tissues“. This enlarged and updated English version aimed exclusively at the...

 
author: Pavel Měřička, Pavel Navrátil, Daniel Brandejs, Lubomír Štěrba, Hana Straková, Barbora Honegrová | LF UK Hr. Králové | discipline: Surgery, Traumatology and Orthopaedics | category: Pear reviewed publications, Elearning courses | keywords: E-learning, programmed instructions, cell and tissue donation, cell and tissue harvest, cell and tissue transplantation, tissue bank, tissue establishment, procurement establishment | attachment: 2 | viewed: 5549x | published on: 20.4.2016 | last modified on: 5.8.2016

Neural development and embryonic stem cell-based developmental modelling

Neural development and embryonic stem cell-based developmental modelling

This educational article titled “Neural development and embryonic stem cell-based developmental modelling“ describes how to investigate the roles of genes in human neural development using stem cell technologies and molecular biology techniques. This educational article is primarily focused on experimental practices. Thus this article is intended for postgraduate students (e.g. Master student, Diploma, and PhD students). However, the article is also suitable for undergraduate students, who are interested in stem cell biology and molecular biology. In the text, students will learn how to perform basic molecular biology techniques (e.g. gene cloning, making fusion protein, PCR and sequencing), and how to grow and to manipulate human embryonic stem cells (hESCs). Moreover, students will learn how to drive hESCs toward to neural fates.

 
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SCHWARZ Daniel, DUŠEK Ladislav. A uniform platform for electronic publishing and sharing the educational content in the network of medical faculties MEFANET [online]. Masaryk University, [2008], [cited 2018-06-22]. Available from WWW: <http://portal.mefanet.cz>. Version 1.9 [2014].