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protein preparation

Protein preparation

This course contains five sub-courses, which consist of theoretical and practical parts. The individual sub-courses should provide students simple and quick approaches needed for solving basic scientific problems that could rise during the course of their bachelor, diploma or PhD study. The first set will allow students to gain knowledge about protein cloning, expression and purification. The second part focuses on biochemical characterization of enzymes via their enzymatic activity as well as protein-protein interactions. The third set will provide simple biochemical methods to detect protein-protein and protein-DNA interactions as well as monitor motor activity of protein.

author: Lumír Krejčí | LF MU | discipline: Biophysics, Medical Chemistry and Biochemistry | category: Educational works | keywords: Protein-protein and protein-DNA interaction methods, EMSA, pull-down, yeast 2 hybrid, Helicase assay, sumoylation | attachment: 5 | viewed: 3695x | published on: 18.6.2012 | last modified on: 20.8.2013

RESULTS FOUND IN FULLTEXT: number of results: 11 | SANDBOX - number of results: 3

Neural development and embryonic stem cell-based developmental modelling
.. biology techniques (e.g. gene cloning, making fusion protein, PCR and sequencing), and how to grow.. .. techniques (e.g. gene cloning, making fusion protein, PCR and sequencing), and how to grow and to.. ..keywords: cloning, fusion protein, PCR, cDNA, cloning vectors, sequencing,.. ..keywords: cloning, fusion protein, PCR, cDNA, cloning vectors, sequencing, human..

Molecular biology
.. 18.6.2012 210.39 KB MEFANET user – Courses Protein Preparation and Characterization III -.. ..keywords: ligation, transformation, plasmid preparation, protein purification, chromatography, sumoylation,..

Analysis of genomic and proteomic data
.. from pre-processing through differential gene(protein)-expression to pathways and meta-analysis of..

Hypertext atlas of Dermatopathology
.. individual image parts, shading compensation and fusion of the image parts into one large image. The..

Návody k praktickým cvičením z molekulární biologie
.. se na dědičnosti organizmů, tedy DNA, RNA a proteinům, jejich vzájemné interakci a regulaci jejich fu..

Dechový test s močovinou značenou uhlíkem 13C ( 13C-UBT)
.. enzymem, ureázou, která je jako povrchový protein produkována bakterií Helicobacter pylori. Metoda ..

B lymphocytes and plasma cells
.. Ig requires its association with other two proteins, called Ig alpha and Ig beta, which function in..

Nekonvenčné T-lymfocyty
.. molekulou sú MR1-molekuly (MHC-related protein 1). MR1 je evolučne konzervovaný nepolymorfný pr..

Acute abdomen - gallstone ileus
.. Patient was without anaemia. C- reactive protein (57 mg/ml), total bilirubin (42 mg/dl) and..

Náhla príhoda brušná - biliárny ileus
.. celkový bilirubín (42 mg/dl) a C reaktívny proteín (57 mg/ml). Zobrazovacie metódy: Na natívnej s..

Podtlakové uzatváranie rán – nové poznatky a možnosti aplikácie
.. z faktorov hojenia je hladina matrixových metaloproteináz (MMP), ktorých je v chronických ranách nad..

SCHWARZ Daniel, DUŠEK Ladislav. A uniform platform for electronic publishing and sharing the educational content in the network of medical faculties MEFANET [online]. Masaryk University, [2008], [cited 2019-11-19]. Available from WWW: <http://portal.mefanet.cz>. Version 1.9 [2014].