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Neural development and embryonic stem cell-based developmental modelling

Neural development and embryonic stem cell-based developmental modelling

This educational article titled “Neural development and embryonic stem cell-based developmental modelling“ describes how to investigate the roles of genes in human neural development using stem cell technologies and molecular biology techniques. This educational article is primarily focused on experimental practices. Thus this article is intended for postgraduate students (e.g. Master student, Diploma, and PhD students). However, the article is also suitable for undergraduate students, who are interested in stem cell biology and molecular biology. In the text, students will learn how to perform basic molecular biology techniques (e.g. gene cloning, making fusion protein, PCR and sequencing), and how to grow and to manipulate human embryonic stem cells (hESCs). Moreover, students will learn how to drive hESCs toward to neural fates.


B lymphocytes and plasma cells

B lymphocytes and plasma cells

B lymphocytes are principal cells that mediate humoral adaptive immunity. After their maturation in the bone marrow, B cells enter peripheral lymphoid tissues, which are the sites of interaction with foreign antigens. Production of antibodies is initiated by the interaction of antigens with a small number of mature B cells specific for each antigen. An antigen binds to the membrane receptors on specific B cells and initiates a series of responses that lead to two principal changes: cell proliferation resulting in expansion of the clone, and differentiation to either plasma cells actively secreting antibodies or to memory cells.

Key words: B cells, subsets of B cells, memory B cells, plasma cells

author: Milan Buc | LF UK v Bratislave | discipline: Immunology, Allergology | category: Lectures, Presentations | keywords: B cells, B cell subsets, memory B cells, plasma cells | attachment: 1 | viewed: 1022x | published on: 6.3.2017



The design of the e-learning course: Donation and Harvest of cells and tissues“ is presented. This course represents more advanced updated and enlarged English version of the original e-learning course focused exclusively on the issues of cell and tissue donation and harvest.
The course covers the topic Donation and harvest of cells and tissues and is divided into 2 parts. A test consisting of 5 questions follows each part. If the student answers correctly at least 3 questions he/she is allowed to pass to the next part and/or final test consisting of 15 questions. It is necessary to answer correctly at least 10 questions to complete the course.
The third topic is represented by a database for uploading seminar theses.
The previous experience showed that the e-learning course could serve as a useful support of the elective subject „Basis of Harvest and Preservation of Tissues“. This enlarged and updated English version aimed exclusively at the topic Donation and harvest of cells and tissues is planed to be used by a broader spectrum of users including the surgeons in post-graduate training and post-graduate students of different doctoral study programmes.

author: Pavel Měřička, Pavel Navrátil, Daniel Brandejs, Lubomír Štěrba, Hana Straková, Barbora Honegrová | LF UK Hr. Králové | discipline: Surgery, Traumatology and Orthopaedics | category: Pear reviewed publications, Elearning courses | keywords: E-learning, programmed instructions, cell and tissue donation, cell and tissue harvest, cell and tissue transplantation, tissue bank, tissue establishment, procurement establishment | attachment: 2 | viewed: 4295x | published on: 20.4.2016 | last modified on: 5.8.2016

RESULTS FOUND IN FULLTEXT: number of results: 12 | SANDBOX - number of results: 6

Pathophysiology practicals for General Medicine and Dental Medicine courses
.. of the possible approaches to study human diseases and their complexity is to use..

Materials for Biophysics
.. Ecology Efects of electric current on human body The Merck manual Hearing Gamma Knife .. .. – – Effects of electric current on human body | 8.11.2010 48 KB registered user –..

Knee joint aspiration
.. that is inflammed can also remove white blood cells, that are sources of enzymes, that can be..

Essentials of Medical Chemistry and Biochemistry
.. conversions of compounds occurring in the human body. The knowledge of presented facts is..

Prezentácia antigénov
.. bunky prezentujúce antigén (antigen presenting cells - APC). Tie pohltený antigén &..

NK- a NKT-bunky - ich charakteristika, biologický a medicínsky význam
.. označujeme ako LAK (lymphokine activated killer cells); im sa podobajú TIL (tumour infiltrating .. .. ovplyvňujú aktivitu iných buniek imunitného systému. Nachádzajú sa najmä v sekundárnych lymfoidných .. .. zabíjačské bunky“ (natural killer cells – NK cells). Štandardne sa zaraďujú k bu..

Anatomical Dissection - Introduction to dissection course
.. they will be able to dissect any region of a human cadaver under supervision of an experienced.. .. structures that students have learnt in the systematic anatomy. The goal of the educational film..

T-lymfocyty a ich subpopulácie
.. Th1, Th2,Th9, Th 17 a Th22. Každý biologický systém zahŕňa v sebe nielen efektorové, ale aj ko.. .. dve subpopulácie –Tcm (T central memory cells) a Tem (T effector memory cells). Gama/delta.. .. alebo indukciou apoptózy. Každý biologický systém zahŕňa v sebe nielen efektorové, ale aj ko.. .. dve subpopulácie –Tcm (T central memory cells) a Tem (T effector memory cells). Tcm-lymfocyty..

Topographical anatomy of limbs
.. anatomy, which is more practical then systematic anatomy and is closer to perception of.. .. a diligent dissection of selected region of the human body, occasionally adjustment of older.. .. relationships. It is more practical then systematic anatomy and is closer to perception of..

TELEHEMATOLOGIE: společné řešení pro edukační obrazový archiv a vzdálené konzultace
.. ImageMagick. Server je vybaven operačním systémem Linux, pro chod archivu jsou dále nutné: A.. .. Sydney Tokyo, 2003. [4] Bain B.: Blood Cells. A Practical Guide. Second Edition, Blackwell..

Epidemiology of malignant tumours in the Czech Republic
.. over and clicking on a specific part of the human body (in case of the schematic diagram) or.. .. of the SVOD project is to develop information systems supporting managerial decision-making and..

Imunopatogenéza a imunoterapia ulceróznej kolitídy
.. infliximab je chimérová a adalimumab zase plne humanizovaná mAb. Na túto liečbu odpovedá asi tretina p.. .. Podobne sa zistilo, že ak bunky imunitného systému exprimujú membránovo viazaný TNF, tak na an.. .. Spits H, Artis D, Colonna M. Innate lymphoid cells — a proposal for uniform nomenclature. Nat..

SCHWARZ Daniel, DUŠEK Ladislav. A uniform platform for electronic publishing and sharing the educational content in the network of medical faculties MEFANET [online]. Masaryk University, [2008], [cited 2018-01-23]. Available from WWW: <http://portal.mefanet.cz>. Version 1.9 [2014].