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Protein preparation

Protein preparation

This course contains five sub-courses, which consist of theoretical and practical parts. The individual sub-courses should provide students simple and quick approaches needed for solving basic scientific problems that could rise during the course of their bachelor, diploma or PhD study. The first set will allow students to gain knowledge about protein cloning, expression and purification. The second part focuses on biochemical characterization of enzymes via their enzymatic activity as well as protein-protein interactions. The third set will provide simple biochemical methods to detect protein-protein and protein-DNA interactions as well as monitor motor activity of protein.

author: Lumír Krejčí | LF MU | discipline: Biophysics, Medical Chemistry and Biochemistry | category: Educational works | keywords: Protein-protein and protein-DNA interaction methods, EMSA, pull-down, yeast 2 hybrid, Helicase assay, sumoylation | attachment: 5 | viewed: 2688x | published on: 18.6.2012 | last modified on: 20.8.2013

RESULTS FOUND IN FULLTEXT: number of results: 13

Molecular biology
.. genetic element such as a bacterial plasmid. The DNA of interest can then be replicated.. .. 210.39 KB MEFANET user – Courses Protein Preparation and Characterization III - Protein-mediated.. ..keywords: PCR, DNA, cloning, DNA ligation, transformation, plasmid preparation, protein purification,.. ..keywords: cloning, DNA ligation, transformation, plasmid preparation, protein purification, chromatography,..

Pathophysiology for Surgery
.. onto clinical subjects. However, during the preparation for the state exam from surgery as well as for..

Blood taking
.. blood. It gives a concise documentation on the preparation of the patient prior to the taking and the..

General and specialized psychiatry
.. psychiatry. They are supposed to be used in your preparation for psychiatry courses and should be helpful.. .. published on these webpages will facilitate your preparation both for the course and the examination, and..

Practicals in Pharmacology
.. forms with examples of individually prepared preparation prescriptions in dermatology,..

Databases in MS Access
.. in following presentations intended for preparation for practical exercises.Prílohy: Príloha Dátum ..

Anatomical Dissection of the Head
.. to provide them with information necessary for preparation to the dissection of the head within the scope..

The Use of the Er:YAG Laser 2940 nm in complex caries removal
.. of the vibrations give advantage to LASER cavity preparation. The ideal treatment should be fast, painless.. .. basis. She was interested to perform cavity preparation using laser instead of conventional approach...

Basics of Nursing Presentations
..keywords: procedures, theoretical introduction, patient preparation, equipment, process,..

Aortocoronary bypass
.. KB MEFANET user Figure 4 - Great saphenous vein preparation. | 22.11.2017 271.1 KB MEFANET user Figure 5 -..

Ascending aorta replacement and repair of aortic valve because of ascending aorta aneurysm and bicuspid aortic valve with regurgitation
.. picture - left common coronary artery preparation. | 22.11.2017 272.78 KB MEFANET user Figure 8 -..

Mitral valve replacement and MAZE procedure because of mitral valve stenosis and paroxysmal atrial fibrillation
.. Figure 5 - Peroperative picture - left atrium preparation. | 22.11.2017 230.26 KB MEFANET user Figure 6 -..

.. principles of this topic and facilitates preparation, presentation and archivation of seminar theses...

SCHWARZ Daniel, DUŠEK Ladislav. A uniform platform for electronic publishing and sharing the educational content in the network of medical faculties MEFANET [online]. Masaryk University, [2008], [cited 2018-01-22]. Available from WWW: <http://portal.mefanet.cz>. Version 1.9 [2014].