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Helicase activity assay

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Process workflow a. Preparation of samples b. Incubation, stop of the reaction c ... Mutations in the BLM gene lead to Bloom’s syndrome, a disorder associated with a predisposition to cancer of all kinds ...

LF MU | discipline: Biophysics | ...: Array | published on: 18. 6. 2012

3. week WT

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On the other hand, a low Km value means a high enzyme activity for a given substrate, i.e., the enzyme preferentially converts such a substrate. 7 ... At 5-minute intervals, transfer 2 drops from each tube into a porcelain plate with wells containing a drop of Lugol solution. 4 ...

UPJŠ LF v Košiciach | discipline: Medical Chemistry and Biochemistry | ...: Array | published on: 16. 9. 2013

2. week WT

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Free phosphate can be detected by a color reaction in an acidic environment with molybdate and ammonium vanadate to give a yellow-green to blue-green coloration ... Gently warm the tube in a boiling water bath for about 10 minutes. 5 ...

UPJŠ LF v Košiciach | discipline: Medical Chemistry and Biochemistry | ...: Array | published on: 15. 2. 2011

NEWMED: New Era in Medical Education

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Students have to complete a home assignment, for example to film a short video or write a case report ... • Asking questions: as in a traditional classroom, students can raise their hand to ask a question and unmute their microphone if they are called upon ...

LF MU | discipline: Other | ...: Array | published on: 14. 9. 2022

12. week ST

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The following three bands were generated by digesting a gene with a single restriction enzyme Eco RI and BamHI ... Consider the following electrophoresis gel. A scientist wishes to isolate a gene that is 1.6 kb in size ...

UPJŠ LF v Košiciach | discipline: Medical Chemistry and Biochemistry | ...: Array | published on: 16. 9. 2013

Venereology part 1. 2.

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... page SyphilisSyphilis –– Lues Lues VenereaVenerea isis a a chronicchronic infectiousinfectious diseasedisease causedcaused byby TreponemaTreponema pallidumpallidum subspeciessubspecies pallidumpallidum –– SpirochetaSpirocheta pallidapallida, , F ... A A smallsmall scarescare afterafter ulcusulcus durumdurum HIV TEST POSITIVEHIV TEST POSITIVE VENEREOLOGY Is syphilis still common infection ??? ...

1.LF UK | discipline: Dermatology | ...: Array | published on: 12. 1. 2007

8. week ST

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Record any observed color changes in the tables and attempt to interpret their significance. A. Model of respiratory acid-base disorders - adjustment using CO2 a HCO3 - In flasks no. 1 and 2, introduce CO2 by inserting a clean glass pipette and blowing air from your lungs into the solution until there is a noticeable change in the indicator color, ensuring consistency between both flasks. Subsequently, eliminate CO2 from the solution in the first flask by introducing atmospheric air into the solution using a pipette with a pump until the color reverts to its original state ...

UPJŠ LF v Košiciach | discipline: Medical Chemistry and Biochemistry | ...: Array | published on: 16. 9. 2013

Disperse systems and transport processes

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... page Viscosity Is the resistance of the fluid to flow Viscosity: • Influences the real liquid flow The causes of viscosity in a fluid: • Intermolecular force of cohesion • Molecular momentum exchange • The layers of liquid move at different speeds, the middle layers have the largest, at the wall the speed is zero – adherese to wall – strong friction ... page Diffusion • Diffusion is a spontaneous process of trnasferring particles from a higher concentration site to a site with a lower concentration until a steady state occures ...

UPJŠ LF v Košiciach | discipline: Biophysics | ...: Array | published on: 17. 3. 2017

3. week WT

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Polysaccharides are detected by color reactions e.g. with iodine (Lugol test) which intercalates into the structure of the carbohydrates. Starch has a characteristic long helical structure, iodine is incorporated into the helix, resulting in a starch-iodine complex that gives a dark blue color ... Prepare solutions in two test tubes according to the data in the table (label test tubes): Test tube Chemicals T1 T2 HCl (mL) 1 1 Phloroglucinol a few crystals a few crystals Heat to boiling (1 min) ...

UPJŠ LF v Košiciach | discipline: Medical Chemistry and Biochemistry | ...: Array | published on: 15. 2. 2011

3. week ST

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The enzyme catalase is a haemoprotein containing 4 molecules of haem ... On contrary, a low Km value means a high enzyme activity for a given substrate, so the enzyme favours the particular substrate. 4 ...

UPJŠ LF v Košiciach | discipline: Medical Chemistry and Biochemistry | ...: Array | published on: 15. 2. 2011

Practical lessons 13

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The third ventricle communicates with the fourth ventricle by a narrow opening, the cerebral aqueduct (of Sylvius). The lateral ventricle is semicircular in shape with a backward directed projection, which extends to the occipital lobe ...

UPJŠ LF v Košiciach | discipline: Anatomy | ...: Array | published on: 30. 6. 2018

3. week ST

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It is synthesized in the liver (and to a lesser extent in the kidneys), released into the blood, and excreted in the urine at a rate of 300 – 550 mmol/day, which corresponds to approximately 14 g of nitrogen. A small amount is also excreted in the feces and sweat ...

UPJŠ LF v Košiciach | discipline: Medical Chemistry and Biochemistry | ...: Array | published on: 16. 9. 2013

4. week WT

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In a solution with a physiological pH (pH = 7.4), FAs are ionized (with deprotonated carboxyl group), causing a negative charge of FAs in living organisms ... Immediately after mixing, transfer 5 mL of mixture into a titration flask and place the residue of mixture in Erlenmeyer flask in a 37 °C water bath ...

UPJŠ LF v Košiciach | discipline: Medical Chemistry and Biochemistry | ...: Array | published on: 15. 2. 2011

Selected infectious diseases of viral or bacterial origin - Measles

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A runny nose, a cough, red and watery eyes, and small white spots inside the cheeks can develop in the initial stage. After several days, a rash erupts, usually on the face and upper neck ...

LF UK Hr. Králové | discipline: Biology | ...: Array | published on: 30. 9. 2015

Laboratory safety rules

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In the laboratory a student must not drink, eat, smoke, chew a chewing gum, do work which is not in the program of laboratory exercise, take drugs or pills decreasing attention ... In case of a bigger fire we will use a fire extinguisher. 15 ...

UPJŠ LF v Košiciach | discipline: Medical Chemistry and Biochemistry | ...: Array | published on: 15. 2. 2011

Hypoventilačný syndróm pri obezite

Hypoventilačný syndróm pri obezite

E66.22 Extrémna obezita s alveolovou hypoventiláciou BMI: 40 a viac ...

UPJŠ LF v Košiciach | discipline: Internal Medicine | keywords: E66.22 Extrémna obezita s alveolovou hypoventiláciou BMI: 40 a viac, J96.11 Chronická respiračná insuficiencia nezatriedená inde typ II (hyperkapnický), G47.31 Obštrukčné spánkové apnoe, obezita, hypoventilácia, respiračné zlyhanie, neinvazívna ventilácia | published on: 22. 12. 2018

Tables

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• description (easier orientation in a table while entering the data) DESIGN OF TABLE page • properties of table fields • field size (length of character string/size of entered number) • format (format for viewing and printing) • input mask (better way to enter data) • caption (field description, more extensive definition of field ... • indexed (creating an index – faster searching a sorting of records) • intelligent marks (adding an intelligent mark to make work with a table easier) DESIGN OF TABLE page main/primary key • field, that guaranties an unique identification of each record • field of primary key guarantees, that there are no equal records in whole table • purpose of a primary key is to prevent duplicate records PRIMARY KEY page • relation database – interconnection of tables • relation (relationship): • tables have no relation (the tables consist of data that have no relation) • tables have relation one to one (one record in one table has just one related record in another table) • tables have relation one to many (one record in one table has several records in another table) • tables have relation many to many (more record in one table relate to more record in another table) RELATIONS page enforce referential integrity • enhanced error checking, which prevents users from making a change that violates the rules of a relationship • ensure that related data is always consistent cascade update related fields • change of value in record of first table will cause an actualisation of corresponding records in related table cascade delete related records • removal of the record in first table will cause a deletion of corresponding records in related table Use carefully! ...

UPJŠ LF v Košiciach | discipline: Medical Informatics and Information Science | ...: Array | published on: 28. 11. 2010

5. week WT

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It is a homogenate of muscle tissue in which the proteins are precipitated by saturation of the solution with ammonium sulphate, (NH4)2SO4. 1.1 Detection of fructose-1,6-bisphosphate Principle: Fructose-1,6-bisphosphate (a hexose-phosphate) is hydrolyzed to fructose (a hexose) by hydrochloric acid ... Put the test tubes into a boiling water bath for a short time (max 5 sec.!) ...

UPJŠ LF v Košiciach | discipline: Medical Chemistry and Biochemistry | ...: Array | published on: 16. 9. 2013

Basic concepts and basic terms

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Data Numbers page STATISTICS Statistics field of study concerned with: • collection, organization, summarization, and analysis of data • drawing of inferences about a body of data when only a part of the data is observed page MAIN AREAS OF STATISTICS Statistics Mathematical statistics • development of new methods of statistical inference • requires detailed knowledge of abstract mathematics for its implementation Applied statistics • applying the methods of mathematical statistics to specific subject areas (economics, psychology, public health etc ... • the largest collection of values of a random variable for which we have an interest at a particular time • populations may be finite or infinite • example: weights of all patients with certain disease Sample • a part of a population (a portion of an entire population) • example: weights of several patients with certain disease BASIC TERMS page Thank you for your attention Jaroslav Majernik jaroslav.majernik@upjs.sk ...

UPJŠ LF v Košiciach | discipline: Medical Informatics and Information Science | ...: Array | published on: 11. 12. 2009

6. week ST

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The resulting pyruvate reacts with a solution of 2,4-dinitrophenylhydrazine to form the pyruvate hydrazone. The ALT determination method is based on measuring the absorbance of the pyruvate hydrazone, which has a reddish-brown color (A 505 nm) ...

UPJŠ LF v Košiciach | discipline: Medical Chemistry and Biochemistry | ...: Array | published on: 16. 9. 2013

Medical sexology

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BIOLOGICAL THERAPY A – R E V E R S I B L E HORMONAL (CYPROTERON ACETATE – ANDROCUR MEDROXYPROGESTERONACETATE – DEPO – PROVERA slide slide-master-content slide-content TREATMENT OF PARAPHILIAS 2. BIOLOGICAL THERAPY A – R E V E R S I B L E NEUROLEPTICS (DEPOT FORMS) THYMOPROPHYLACTIS (LITHIUM, CARBAMAZEPINE) SSRI (EXPERIMENTAL) slide slide-master-content slide-content TREATMENT OF PARAPHILIAS 2 ...

LF MU | discipline: Psychiatry, Psychology, Sexology | ...: Array | published on: 12. 1. 2006

BLS in special circumstances

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... date 2011-02-10T20:33:17Z pdf:PDFVersion 1.5 xmp:CreatorTool Microsoft® Publisher 2010 stream_content_type application/pdf pdf:hasXFA false access_permission:modify_annotations true access_permission:can_print_degraded true dc:creator language cs-CZ dcterms:created 2011-02-10T20:33:17Z Last-Modified 2011-02-10T20:33:17Z dcterms:modified 2011-02-10T20:33:17Z dc:format application/pdf; version=1.5 Last-Save-Date 2011-02-10T20:33:17Z pdf:docinfo:creator_tool Microsoft® Publisher 2010 access_permission:fill_in_form true pdf:docinfo:modified 2011-02-10T20:33:17Z stream_name https://portal.lf1.cuni.cz/_down1lfMefa48s56PRTG554-89898sds/4/160-bls-in-special-circumstances-pdf_4d544d9765049.pdf meta:save-date 2011-02-10T20:33:17Z pdf:encrypted false modified 2011-02-10T20:33:17Z pdf:hasMarkedContent true Content-Type application/pdf stream_size 718732 pdf:docinfo:creator X-Parsed-By org.apache.tika.parser.DefaultParser X-Parsed-By org.apache.tika.parser.pdf.PDFParser creator dc:language cs-CZ meta:author meta:creation-date 2011-02-10T20:33:17Z stream_source_info url created 2011-02-10T20:33:17Z access_permission:extract_for_accessibility true access_permission:assemble_document true xmpTPg:NPages 14 Creation-Date 2011-02-10T20:33:17Z pdf:hasXMP false access_permission:extract_content true access_permission:can_print true Author producer Microsoft® Publisher 2010 access_permission:can_modify true pdf:docinfo:producer Microsoft® Publisher 2010 pdf:docinfo:created 2011-02-10T20:33:17Z page Foreign Body Airway Obstruction  Primary assessment D R s A B C  Get help  Secondary assessment A B C D E  Provide first aid  Reassess regularly  Transport to health care facility Basic Life Support in special circumstances  Foreign Body Airway Obstruction (choking) sequences  Start with primary assessment  If the victim is not responsive and signs of life are not present  start with BLS (CPR)  If the victim is responsive, continue to secondary assessment and start with A  decide if the airway obstruction is present page Basic Life Support in special circumstances  Foreign Body Airway Obstruction (choking) sequences  Decide if the airway obstruction is mild or severe Foreign Body Airway Obstruction  Secondary assessment A B C D E Mild obstruction  ‘‘Are you choking?’’ ‘‘Yes’’  Able to vocalise  Loud cough  Able to take a breath before coughing Severe obstruction  ‘‘Are you choking?’’ ...

1.LF UK | discipline: Health Care Sciences | ...: Array | published on: 10. 2. 2011

Basic terms

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... page STATISTICS – BASIC TERMS variable - characteristic or attribute that can assume different values random variable - a variable whose values are determined by chance qualitative variables - variables which assume non-numerical values quantitative variables - variables which assume numerical values discrete variables -variables which assume a finite or countable number of possible values ... Usually they come from: survey – data represent observations of events or phenomena over which few, if any, controls are imposed experiment – a research plan is designed to purposely impose controls over the amount of treatment (e.g. in clinical trials) ...

UPJŠ LF v Košiciach | discipline: Medical Informatics and Information Science | ...: Array | published on: 9. 10. 2019

ECDL

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• To achieve an ECDL / ICDL certification, the Candidate must  successfully pass a test in all seven modules. page ECDL / ICDL Module 1 is a theoretical test of computing knowledge at a  general level and modules 2‐7 are practical skills tests, as follows:  • Module 1 – Concepts of Information Technology (IT) • Module 2 – Using the Computer and Managing Files • Module 3 –Word Processing • Module 4 – Spreadsheets • Module 5 – Database • Module 6 – Presentation • Module 7 – Information and Communication page • When Candidates register to start the ECDL / ICDL certification  process they purchase a Skills Card on which their progress through  the seven Tests is recorded ... It is  intended for those who need to, or wish to, know how to use a personal  computer.  Benefits for Candidates:  • Increases essential IT knowledge/skills  • Increases confidence in computer use  • Provides internationally–recognised qualification  • Improves job prospects and job mobility  • Provides a platform from which to move on to higher‐level IT education Benefits for Employers:  • Increases productivity  • Reduces costs  • Increases the quality of employee output  • Reduces time wasted  • Makes better use of IT resources  • Provides a better return on IT investment page ...

UPJŠ LF v Košiciach | discipline: Medical Informatics and Information Science | ...: Array | published on: 25. 4. 2008

Diagnosis and Therapy

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In general practice, understanding symptoms is crucial for a successful diagnosis. Up to 50% of patients visiting general practice may not receive a definitive diagnosis based solely on pathological symptoms ... The combination of symptoms and diagnostic findings that confirm a predicted diagnosis is referred to as a syndrome ...

UPJŠ LF v Košiciach | discipline: General Practice Medicine | ...: Array | published on: 10. 2. 2025

10. week WT

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It is a homogenate of muscle tissue in which the proteins are precipitated by saturation of the solution with ammonium sulphate, (NH4)2SO4. 1.1 Detection of fructose-1,6-bisphosphate Fructose-1,6-bisphosphate (a hexose-phosphate) is hydrolyzed to fructose (a hexose) by hydrochloric acid ... Put the test tubes into a boiling water bath for a short time. 3 ...

UPJŠ LF v Košiciach | discipline: Medical Chemistry and Biochemistry | ...: Array | published on: 15. 2. 2011

Selected infectious diseases of viral or bacterial origin - Acute gastroenteritis of viral etiology

docx Selected infectious diseases of viral or bacterial origin - Acute gastroenteritis of viral etiology
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[28] Obinata K, Okumura A, Nakazawa T, Kamata A: Norovirus encephalopathy in a previously healthy child ... [37] Gubbay J, Al-Rezqi A, Hawkes M, Williams L, Richardson S, Matlow A ...

LF UK Hr. Králové | discipline: Biology | ...: Array | published on: 30. 9. 2015

Thinking

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Dept. of Psychiatry, Masaryk University , Brno slide slide-master-content slide-content Thinking definition of thinking thought disorder quantitative disturbances of thinking qualitative disturbances of thinking delusions literature slide slide-master-content slide-content Definition Thinking: Goal-directed flow of ideas and associations initiated by a problem and leading toward a reality-oriented conclusion. Thinking is a very complex and complicated psychic function ...

LF MU | discipline: Psychiatry, Psychology, Sexology | ...: Array | published on: 12. 1. 2006

Selected animations from Anatomy

Selected animations from Anatomy

Vessels of head and neck [00:05:19]  Vessels of upper limb [00:07:27]  Nerves of upper limb [00:07:59]   Vessels of lower limb [00:08:17]  Nerves of lower limb [00:03:26]  Anatomy 1 [UA/A-GM1/14] Anatomy 2 [UA/A-GM2/14] Anatomy 3 [UA/A-GM3/14] Anatomy 1 [UA/A-DM1/15] Anatomy 2 [UA/A-DM2/15] Department of Anatomy Anatomical dissection - upper limb (videos) Anatomical dissection - lower limb (videos) Anatomical dissection - thorax, axilla, respiratory system (videos) Anatomical dissection - abdomen and pelvis (videos) Anatomical dissection - head and neck (videos) ...

UPJŠ LF v Košiciach | discipline: Anatomy | keywords: head, neck, vessels, nerves, upper limb, lower limb, vessels, head, neck | published on: 14. 10. 2011

Esophagus

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... page Peptic  esophageal  ulcer   Some  consider  it  a  single  nosologic  unit,  others  consider  it  a  quantitative  change  of  ulcerous   reflux   esophagitis.   ... Angioma:  it  is  often  a  source  of  bleeding.     page Papilloma:   it   is   usually   around   the   esophageal   sphincters,   it   has   a   typical   look   –   a   pale   surface,  a  typical  shape.     ...

LF MU | discipline: Otorhinolaryngology | ...: Array | published on: 4. 4. 2016


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